Expression of Bitter Taste Receptors in Human Bronchial airways Smooth Muscle Cells by using Immunofluorescence Staining

Abstract

   Asthma and chronic obstructive pulmonary diseases (COPDs) have been identified as one of the serious, public health concerns in the world, which results due to the host as well as the environmental factors. Based on the WHO, this disease will be the 3^rdkiller disease worldwide by the year of 2020 following the cancer and heart disease. Concerning the asthma, there have been 389,000,000 individuals worldwide, afflicted by COPD and Asthma. Moreover, there has been a noticeable increase in the asthmatic incidences in the young adults and children by 4% to 5% yearly worldwide. Those two illnesses are fundamental global morbidity and mortality reasons, particularly in the patients that respond poorly to the current treatments. Which is why, there are unmet needs to come up with innovative therapies for the COPD as well as the asthma patients. Bitter taste receptors (TAS2R) are part of the family of the G-protein coupled receptors (GPCR). It was theorized previously that the TAS2R only expression on the tongue’s taste buds. The family of the TAS2R includes over 25 different subtypes of the receptors in the humans. Lately, a number of the researches showed that the TAS2R could be representing a new treatment target of the lung diseases. The TAS2R has been expressed in the human air-way smooth muscles (ASMs). It should be noted that TAS2Ractivation by the saccharin and chloroquine results in the induction of air-way relaxation. More significantly, bronchodilatory effects that result from the TAS2R agonists has been found greater compared to the β2 adrenergic receptor agonists, the basic bronchodilator medications in treating asthma. the bronchial airway smooth muscle (BASM) cells have been cultured from COPD (n=3), asthmatic (n=3), and healthy (n=3) individuals. The Immuno-fluorescence staining (with and with no permeabilize cells) have been conducted on 3 groups with the use of the poly-clonal anti-bodies against the (TAS2R10 as well as the TAS2R14) receptors. The immuno-fluorescence assays had shown that the TAS2R10 and TAR2R14 had shown a positive staining of the two receptors in the human ASM cells. It has been discovered as well that those two receptors have been expressed as well in nucleus.